**************** The 19th., Lab Seminar ****************************

Date: Tue, March 20, 2007  PM 1:30-

Place: No.616 Seminar room
　　　　　　 Graduate School of Information Science

Speaker: Takuya Okamoto

Title: Free radical EPR spectroscopy analysis using blind source separation
[J.Y. Ren et al. / Journal of Magnetic Resonance 166 (2004) 82-91]

Abstract:
 In this paper, we propose a novel approach for electron paramagnetic 
resonance (EPR) mixture spectra analysis based on blind source 
separation (BSS) technique. EPR spectrum of a free radical is often 
superimposed by overlapping spectra of other species. It is important 
and challenging to accurately identify and quantify the ‘pure’ 
spectra from such mixtures. In this study, an automated BSS method 
implementing independent component analysis is used to extract the 
components from mixed EPR spectra that contain overlapping components 
of different paramagnetic centers. To apply this method, there is no 
requirement to know the component spectra or the number of components 
in advance. The method is applied to analyze free radical EPR spectra 
which are collected from standard chemical system, cultured cell 
suspense, and ex vivo rat kidneys by spin trapping EPR technique. 
Results show that the BSS method proposed here is capable of 
identifying the component EPR spectra from mixtures with unknown 
compositions. The BSS technique can offer powerful aids in resolving 
spectral overlapping problems in general EPR spectroscopy analysis.

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